Projects funded by the NCN


Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Nucleoprotein complexes in DNA replication, proteolysis and plasmid post segregational killing

2012/04/A/NZ1/00048

Keywords:

DNA replication plasmid biology proteolysis plasmid RK2

Descriptors:

  • NZ1_1: Molecular biology
  • NZ1_2: Biochemistry
  • NZ1_10: Microbiology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

Uniwersytet Gdański, Międzyuczelniany Wydział Biotechnologii Uniwersytetu Gdańskiego i Gdańskiego Uniwersytetu Medycznego

woj. pomorskie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Igor Konieczny 

Number of co-investigators in the project: 7

Call: MAESTRO 2 - announced on 2011-12-15

Amount awarded: 2 541 800 PLN

Project start date (Y-m-d): 2012-09-21

Project end date (Y-m-d): 2018-03-20

Project duration:: 66 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. wytrząsarka laboratoryjna kołyskowa.
  2. Zestaw chromatograficzny AKTA Prime Plus (80 000 PLN)
  3. wytrząsarka laboratoryjna o ruchu obrotowym.
  4. Szybkoobrotowa wirówka z chłodzeniem z rotorem kątowym z wyposażeniem, (20 000 PLN)
  5. Wysokoobrotowa wirówka z rotorem kątowym Sigma1-14.
  6. bloki grzejne dwumiejscowe.
  7. zestaw do elektroforezy pionowej białek i DNA.
  8. System do dokumentacji materiałów znakowanych chemiluminescencyjnie, fluorescencyjnie i kalorymetrycznie ze sprzętem komputerowym (120 000 PLN)

Information in the final report

  • Publication in academic press/journals (8)
  • Book publications / chapters in book publications (1)
  1. Plasmid replication initiator interactions with origin 13-mers and polymerase subunits contribute to strand-specific replisome assembly.
    Authors:
    Aleksandra Wawrzycka, Marta Gross, Anna Wasaznik, and Igor Konieczny
    Academic press:
    PNAS (Proceedings of National Academy of Sciences) (rok: 2015, tom: x, strony: E4188 -E4196), Wydawca: United States National Academy of Sciences
    Status:
    Published
    DOI:
    10.1073/pnas/150496112 - link to the publication
  2. Proteolysis in plasmid DNA stable maintenance in bacterial cells
    Authors:
    Anna Karlowicz , Katarzyna Wegrzyn , Andrzej Dubiel, Malgorzata Ropelewska, Igor Konieczny
    Academic press:
    Plasmid (rok: 2016, tom: 86, strony: 45486), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.plasmid.2016.05.002 - link to the publication
  3. Replisome Assembly at Bacteria Chromosomes and Iteron Plasmids
    Authors:
    Katarzyna E.Wegrzyn , MartaGross , Urszula Uciechowska and Igor Konieczny
    Academic press:
    Frontiers in Molecular Biosciences (rok: 2016, tom: 3, strony: 45308), Wydawca: Frontiers in Molecular Biosciences
    Status:
    Published
    DOI:
    10.3389/fmolb.2016.00039 - link to the publication
  4. Sequence-specific interactions of Rep proteins with ssDNA in the AT-rich region of the plasmid replication orogin.
    Authors:
    Katarzyna Wegrzyn, Maria Eugenia Fuentes-Perez, Katarzyna Bury, Magdalena Rajewska, Fernando Moreno-Herrero, and Igor Konieczny
    Academic press:
    Nucleic Acids Research (rok: 2014, tom: vol.42, No 12, strony: 7807-7818), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gku453 - link to the publication
  5. ClpAP protease is a universal factor that activates the parDE toxin-antitoxin system from a broad host range RK2 plasmid.
    Authors:
    Dubiel A, Wegrzyn K, Kupinski AP, Konieczny I.
    Academic press:
    Scientific Reports (rok: 2018, tom: 8, strony: 45303), Wydawca: Nature
    Status:
    Published
    DOI:
    10.1038/s41598-018-33726-y - link to the publication
  6. Defining the crucial domain and amino acid residues in bacterial Lon protease for DNA binding and processing of DNA-interacting substrates
    Authors:
    Anna Karlowicz, Katarzyna Wegrzyn, Marta Gross, Dagmara Kaczynska, Malgorzata Ropelewska, Małgorzata Siemiątkowska, Janusz M. Bujnicki and Igor Konieczny
    Academic press:
    Journal of Biological Chemistry (rok: 2017, tom: vol. 292, No 18, strony: 7507-7518), Wydawca: American Society for Biochemistry and Molecular Biology
    Status:
    Published
    DOI:
    10.1074/jbc.M116.766709 - link to the publication
  7. Two replication initiators - one mechanism for replication origin opening?
    Authors:
    Elzbieta Zabrocka, Katarzyna Wegrzy, Igor Konieczny
    Academic press:
    Plasmid (rok: 2014, tom: 76, strony: 72-78), Wydawca: Elsevier
    Status:
    Published
  8. Handcuffing reversal is facilitated by proteases and replication initiator monomers
    Authors:
    Katarzyna Bury, Katarzyna Węgrzyn, Igor Konieczny
    Academic press:
    Nucleic Acids Research (rok: 2017, tom: vol. 45, No 7, strony: 3953-3966), Wydawca: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkx166 - link to the publication
  1. Iteron Plasmids
    Authors:
    Igor Konieczny, Katarzyna Bury, Aleksandra Wawrzycka, Katarzyna Wegrzyn
    Book:
    Microbiology Spectrum (rok: 2014, tom: vol. 2 No 6, strony: 45307), Wydawca: American Society for Microbiology Press
    Status:
    Published