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Nucleoprotein complexes in DNA replication, proteolysis and plasmid post segregational killing

2012/04/A/NZ1/00048

Keywords

Descriptors

  • NZ1_1: Biologia molekularna
  • NZ1_2: Biochemia
  • NZ1_10: Biologia drobnoustrojów

Panel

NZ1 – Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution

Uniwersytet Gdański, Międzyuczelniany Wydział Biotechnologii Uniwersytetu Gdańskiego i Gdańskiego Uniwersytetu Medycznego

Voivodeship: pomorskie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Igor Piotr Konieczny

Number of co-investigators in the project: 7

Call

MAESTRO 2 – announced on 2011-12-15

Amount awarded
2 541 800 PLN
Project start date
2012-09-21
Project end date
2018-03-20
Project duration
66 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. System do dokumentacji materiałów znakowanych chemiluminescencyjnie, fluorescencyjnie i kalorymetrycznie ze sprzętem komputerowym. Cost: 120 000 PLN
  2. Szybkoobrotowa wirówka z chłodzeniem z rotorem kątowym z wyposażeniem,. Cost: 20 000 PLN
  3. Wysokoobrotowa wirówka z rotorem kątowym Sigma1-14.
  4. Zestaw chromatograficzny AKTA Prime Plus. Cost: 80 000 PLN
  5. bloki grzejne dwumiejscowe.
  6. wytrząsarka laboratoryjna kołyskowa.
  7. wytrząsarka laboratoryjna o ruchu obrotowym.
  8. zestaw do elektroforezy pionowej białek i DNA.

Information in the final report

Publication in academic press/journals (8)

  1. Handcuffing reversal is facilitated by proteases and replication initiator monomers

    Authors:
    Katarzyna Bury, Katarzyna Węgrzyn, Igor Konieczny
    Academic press:
    Nucleic Acids Research (year: 2017, volume: vol. 45, No 7, pages: 3953-3966), Publisher: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gkx166 – link to the publication

  2. Defining the crucial domain and amino acid residues in bacterial Lon protease for DNA binding and processing of DNA-interacting substrates

    Authors:
    Anna Karlowicz, Katarzyna Wegrzyn, Marta Gross, Dagmara Kaczynska, Malgorzata Ropelewska, Małgorzata Siemiątkowska, Janusz M. Bujnicki and Igor Konieczny
    Academic press:
    Journal of Biological Chemistry (year: 2017, volume: vol. 292, No 18, pages: 7507-7518), Publisher: American Society for Biochemistry and Molecular Biology
    Status:
    Published
    DOI:
    10.1074/jbc.M116.766709 – link to the publication

  3. Replisome Assembly at Bacteria Chromosomes and Iteron Plasmids

    Authors:
    Katarzyna E.Wegrzyn , MartaGross , Urszula Uciechowska and Igor Konieczny
    Academic press:
    Frontiers in Molecular Biosciences (year: 2016, volume: 3, pages: 46039), Publisher: Frontiers in Molecular Biosciences
    Status:
    Published
    DOI:
    10.3389/fmolb.2016.00039 – link to the publication

  4. Plasmid replication initiator interactions with origin 13-mers and polymerase subunits contribute to strand-specific replisome assembly.

    Authors:
    Aleksandra Wawrzycka, Marta Gross, Anna Wasaznik, and Igor Konieczny
    Academic press:
    PNAS (Proceedings of National Academy of Sciences) (year: 2015, volume: x, pages: E4188 -E4196), Publisher: United States National Academy of Sciences
    Status:
    Published
    DOI:
    10.1073/pnas/150496112 – link to the publication

  5. Sequence-specific interactions of Rep proteins with ssDNA in the AT-rich region of the plasmid replication orogin.

    Authors:
    Katarzyna Wegrzyn, Maria Eugenia Fuentes-Perez, Katarzyna Bury, Magdalena Rajewska, Fernando Moreno-Herrero, and Igor Konieczny
    Academic press:
    Nucleic Acids Research (year: 2014, volume: vol.42, No 12, pages: 7807-7818), Publisher: Oxford University Press
    Status:
    Published
    DOI:
    10.1093/nar/gku453 – link to the publication

  6. Proteolysis in plasmid DNA stable maintenance in bacterial cells

    Authors:
    Anna Karlowicz , Katarzyna Wegrzyn , Andrzej Dubiel, Malgorzata Ropelewska, Igor Konieczny
    Academic press:
    Plasmid (year: 2016, volume: 86, pages: 46216), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.plasmid.2016.05.002 – link to the publication

  7. ClpAP protease is a universal factor that activates the parDE toxin-antitoxin system from a broad host range RK2 plasmid.

    Authors:
    Dubiel A, Wegrzyn K, Kupinski AP, Konieczny I.
    Academic press:
    Scientific Reports (year: 2018, volume: 8, pages: 46034), Publisher: Nature
    Status:
    Published
    DOI:
    10.1038/s41598-018-33726-y – link to the publication

  8. Two replication initiators - one mechanism for replication origin opening?

    Authors:
    Elzbieta Zabrocka, Katarzyna Wegrzy, Igor Konieczny
    Academic press:
    Plasmid (year: 2014, volume: 76, pages: 72-78), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.plasmid.2014.10.003 – link to the publication

Book publications / chapters in book publications (1)

  1. Iteron Plasmids

    Authors:
    Igor Konieczny, Katarzyna Bury, Aleksandra Wawrzycka, Katarzyna Wegrzyn
    Book:
    Microbiology Spectrum (year: 2014, volume: vol. 2 No 6, pages: 46038), Publisher: American Society for Microbiology Press
    Status:
    Published
    DOI:
    link to the publication

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