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Information on the principal investigator and host institution

Information of the project and the call

Keywords

Equipment

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Adaptation mechanisms of conjugative RA3 plasmid from IncU incompatibility group responsible for ability to replicate and stable maintenance in the broad range of bacterial hosts

2011/03/B/NZ1/06540

Keywords:

Broad host range plasmid replication conjugation stable inheritance regulatory network adaptation mechanisms

Descriptors:

  • NZ1_1: Molecular biology
  • NZ1_10: Microbiology

Panel:

NZ1 - Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution :

INSTYTUT BIOCHEMII I BIOFIZYKI PAN

woj. mazowieckie

Other projects carried out by the institution 

Principal investigator (from the host institution):

prof. Grażyna Jagura-Burdzy 

Number of co-investigators in the project: 4

Call: OPUS 2 - announced on 2011-09-15

Amount awarded: 589 300 PLN

Project start date (Y-m-d): 2012-08-02

Project end date (Y-m-d): 2016-02-01

Project duration:: 42 months (the same as in the proposal)

Project status: Project settled

Equipment purchased [PL]

  1. Piec hybrydyzacyjny (12 000 PLN)
  2. Blok grzewczy (2 103 PLN)
  3. Cieplarka - inkubator termiczny stołowy (17 000 PLN)
  4. Mikrowirówka z chłodzeniem Eppendorf.
  5. Termomixer (15 000 PLN)
  6. Zestaw do elektroforezy stołowej- aparat , zasilacz (6 000 PLN)
  7. Kaseta z ekranem do Phosphorimagera (7 500 PLN)
  8. Laptop (8 000 PLN)
  9. Mikrowirówka MiniSpin (3 500 PLN)
  10. Wirówka stołowa (38 000 PLN)
  11. Mieszadło Vortex (1 396 PLN)

Information in the final report

  • Publication in academic press/journals (5)
  1. Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria
    Authors:
    Bartosik, A.A., Glabski, K., Kulinska, A., Lewicka, E., Godziszewska, J., Markowska, A., Jagura-Burdzy G.
    Academic press:
    BMC Microbiology (rok: 2016, tom: 16, strony: 59-74), Wydawca: BioMedCentral
    Status:
    Published
    DOI:
    10.1186/s12866-016-0674-y - link to the publication
  2. Global transcriptional regulation of backbone genes in broad-host-range plasmid RA3 from IncU group involves KorB (ParB) segregation protein.
    Authors:
    Kulinska A, Godziszewska J, Wojciechowska A, Ludwiczak M, Jagura-Burdzy G.
    Academic press:
    Appl Environ Microbiol. (rok: 2016, tom: 82, strony: 2320-2335), Wydawca: American Society of Microbiology (ASM)
    Status:
    Published
    DOI:
    10.1128/AEM.03541-15 - link to the publication
  3. Global transcriptional regulator KorC coordinates expression of three backbone modules of the broad-host-range RA3 plasmid from IncU incompatibility group.
    Authors:
    Ludwiczak M, Dolowy P, Markowska A, Szarlak J, Kulinska A, Jagura-Burdzy G.
    Academic press:
    Plasmid (rok: 2013, tom: 70, strony: 131-145), Wydawca: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.plasmid.2013.03.007 - link to the publication
  4. MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer.
    Authors:
    Godziszewska J., Kulińska A., Jagura Burdzy G.
    Academic press:
    BMC Microbiology (rok: 2014, tom: 14, strony: 235-252), Wydawca: BioMedCentral
    Status:
    Published
    DOI:
    10.1186/s12866-014-0235-1 - link to the publication
  5. Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation.
    Authors:
    Godziszewska, J., Moncalián, G., Cabezas, M., Bartosik, A.A., de la Cruz, F., Jagura-Burdzy, G.
    Academic press:
    Molecular Microbiology (rok: 2016, tom: 101, strony: 439–456), Wydawca: International Union of Microbiological Societies, Wiley
    Status:
    Published
    DOI:
    10.1111/mmi.13401 - link to the publication

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