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Adaptation mechanisms of conjugative RA3 plasmid from IncU incompatibility group responsible for ability to replicate and stable maintenance in the broad range of bacterial hosts

2011/03/B/NZ1/06540

Keywords

Descriptors

  • NZ1_1: Biologia molekularna
  • NZ1_10: Biologia drobnoustrojów

Panel

NZ1 – Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution

Instytut Biochemii i Biofizyki PAN

Voivodeship: mazowieckie

Other projects carried out by the institution »

Principal investigator (from the host institution)

prof. Grażyna Teresa Jagura-Burdzy

Number of co-investigators in the project: 4

Call

OPUS 2 – announced on 2011-09-15

Amount awarded
589 300 PLN
Project start date
2012-08-02
Project end date
2016-02-01
Project duration
42 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. Blok grzewczy. Cost: 2 103 PLN
  2. Cieplarka - inkubator termiczny stołowy. Cost: 17 000 PLN
  3. Kaseta z ekranem do Phosphorimagera. Cost: 7 500 PLN
  4. Laptop. Cost: 8 000 PLN
  5. Mieszadło Vortex. Cost: 1 396 PLN
  6. Mikrowirówka MiniSpin. Cost: 3 500 PLN
  7. Mikrowirówka z chłodzeniem Eppendorf.
  8. Piec hybrydyzacyjny. Cost: 12 000 PLN
  9. Termomixer. Cost: 15 000 PLN
  10. Wirówka stołowa. Cost: 38 000 PLN
  11. Zestaw do elektroforezy stołowej- aparat , zasilacz. Cost: 6 000 PLN

Information in the final report

Publication in academic press/journals (5)

  1. Concerted action of NIC relaxase and auxiliary protein MobC in RA3 plasmid conjugation.

    Authors:
    Godziszewska, J., Moncalián, G., Cabezas, M., Bartosik, A.A., de la Cruz, F., Jagura-Burdzy, G.
    Academic press:
    Molecular Microbiology (year: 2016, volume: 101, pages: 439–456), Publisher: International Union of Microbiological Societies, Wiley
    Status:
    Published
    DOI:
    10.1111/mmi.13401 – link to the publication

  2. Global transcriptional regulator KorC coordinates expression of three backbone modules of the broad-host-range RA3 plasmid from IncU incompatibility group.

    Authors:
    Ludwiczak M, Dolowy P, Markowska A, Szarlak J, Kulinska A, Jagura-Burdzy G.
    Academic press:
    Plasmid (year: 2013, volume: 70, pages: 131-145), Publisher: Elsevier
    Status:
    Published
    DOI:
    10.1016/j.plasmid.2013.03.007 – link to the publication

  3. Global transcriptional regulation of backbone genes in broad-host-range plasmid RA3 from IncU group involves KorB (ParB) segregation protein.

    Authors:
    Kulinska A, Godziszewska J, Wojciechowska A, Ludwiczak M, Jagura-Burdzy G.
    Academic press:
    Appl Environ Microbiol. (year: 2016, volume: 82, pages: 2320-2335), Publisher: American Society of Microbiology (ASM)
    Status:
    Published
    DOI:
    10.1128/AEM.03541-15 – link to the publication

  4. Convenient broad-host-range unstable vectors for studying stabilization cassettes in diverse bacteria

    Authors:
    Bartosik, A.A., Glabski, K., Kulinska, A., Lewicka, E., Godziszewska, J., Markowska, A., Jagura-Burdzy G.
    Academic press:
    BMC Microbiology (year: 2016, volume: 16, pages: 59-74), Publisher: BioMedCentral
    Status:
    Published
    DOI:
    10.1186/s12866-016-0674-y – link to the publication

  5. MobC of conjugative RA3 plasmid from IncU group autoregulates the expression of bicistronic mobC-nic operon and stimulates conjugative transfer.

    Authors:
    Godziszewska J., Kulińska A., Jagura Burdzy G.
    Academic press:
    BMC Microbiology (year: 2014, volume: 14, pages: 235-252), Publisher: BioMedCentral
    Status:
    Published
    DOI:
    10.1186/s12866-014-0235-1 – link to the publication

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