Biochemical, structural and functional characterization of the plant exosome complex, using Arabidopsis thaliana as a model organism

2011/01/D/NZ1/03510

Keywords

Descriptors

  • NZ1_1: Biologia molekularna
  • NZ1_2: Biochemia

Panel

NZ1 – Molecular biology, structural biology, biotechnology: molecular biology, structural biology, biotechnology

Host institution

Instytut Biochemii i Biofizyki PAN

Voivodeship: mazowieckie

Other projects carried out by the institution »

Principal investigator (from the host institution)

dr Rafał Tomecki

Number of co-investigators in the project: 8

Call

SONATA 1 – announced on 2011-03-15

Amount awarded
1 000 000 PLN
Project start date
2011-12-28
Project end date
2016-12-27
Project duration
60 months (the same as in the proposal)
Project status
Project settled

Equipment purchased [PL]

  1. Aparaty do elektroforezy (2 pcs). Cost: 10 000 PLN
  2. Kasety i ekrany do fosfoobrazowania (7 pcs). Cost: 28 000 PLN
  3. Komercyjne kolumny chromatograficzne do sączenia molekularnego oraz chromatografii jonowymiennej (2 pcs). Cost: 20 000 PLN
  4. Komputer (2 pcs). Cost: 10 000 PLN
  5. Pipety automatyczne, pipety elektroniczne, pipetory (18 pcs). Cost: 20 000 PLN
  6. Wirówka z chłodzeniem. Cost: 12 000 PLN

Information in the final report

Publication in academic press/journals (1)

  1. hUTP24 is essential for processing of the human rRNA precursor at site A1, but not at site A0

    Authors:
    Tomecki Rafał, Łabno Anna, Drążkowska Karolina, Cysewski Dominik, Dziembowski Andrzej
    Academic press:
    RNA Biology (year: 2015, volume: 12(9), pages: 1010-1029), Publisher: Taylor & Francis
    Status:
    Published
    DOI:
    10.1080/15476286.2015.1073437 – link to the publication

Book publications / chapters in book publications (1)

  1. Purification of eukaryotic exoribonucleases following heterologous expression in bacteria and analysis of their biochemical properties by in vitro enzymatic assays

    Authors:
    Tomecki Rafał, Drążkowska Karolina, Krawczyk Antonina, Kowalska Katarzyna, Dziembowski Andrzej
    Book:
    RNA remodelling proteins: methods and protocols (year: 2015, volume: 1259, pages: 417-452), Publisher: Springer
    Status:
    Published
    DOI:
    link to the publication

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